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KMID : 0603519990040010034
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Journal of Korean Association of Cancer Prevention
1999 Volume.4 No. 1 p.34 ~ p.43
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Changes of Interleukin-2, 4, 10 in Sarcoma 180-transplanted Mice
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Lee Won-Ki
Kim Kwang-Hyuk
Jeong Yong-Kee
Park Kun-Young
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Abstract
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Experiments were undertaken to investigate the effect of cancer cell, sarcoma 180 (S180) on production of interleukin(IL)-2, 4, and 10 in BALB/C mice. Splenocytes of normal mice were incubated with S 180 cells. The culture supernatant were collected at 2,4, 6,24, and 72 hr and assessed for IL-2,4, and IL-10 production by enzyme-linked immunosorbent assay. The production of IL-2 in the culture supernatant of adherent cells exposed with S 180 was not detected, but it increased at 24 and 72 hr in culture with non-adherent or whole splenocyte. The production of IL-4 in the culture supernatant of S 180, adherent, non-adherent, or whole splenocyte was detected, but by co-culture with S 180 it showed significant increase at 72 hr. Also, the production of IL-10 in the culture supernatant of S 180, adherent, non-adherent, or whole splenocytes was detected. By exposure with S 180 it increased to 10-80 times in comparison with the control. Normal mice were transplanted with S 180 into peritoneum. Sera and peritoneal fluids(PF) were collected at 2, 4, 6, and 24 hr. The production of IL-2 in sera was not detected, but in PF it was a little. The production of IL-4 in sera or PF was detected, but it showed the significant decrease in comparison with the control. The production of IL-10 in sera was detected in transplantation or control group, and it increased in transplantation group. The production of IL-10 in PF showed the increase at 24 hr. By exposure of S 180 tumor cells in immuno-competent cells or transplantation with S 180 into mouse, IL-4 decreased in sera and PF, but IL-10 showed the remarkable increase in the culture supernatant of splenocytes and in the sera. These data suggest that the unbalanced conditions such as suppression of IL-4 production and overproduction of IL-10 if vivo may be responsible for dysfunction of immune system.
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KEYWORD
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Mouse, Sarcoma 180, Interleukin-2, Interleukin-4, Interleukin-10
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